james carlyle
James CarlylePhD
Associate Professor

Contact Info

T. (416) 480-6100
F. (416) 480-5703

Location

Sunnybrook Research Institute
2075 Bayview Avenue, Room S236
Toronto
ON, M4N3M5

Research Interests

Innate Immunity, Developmental Immunology, Infectious Diseases, Cancer Immunology, NK-cells

Accepting

Grad Students Must First Apply Through Department

Appointments

Senior Scientist, Sunnybrook Research Institute

The innate immune system protects us from infection and cancer. Natural killer (NK) cells are a subset of innate lymphoid cells (ILC) that can detect and eliminate transformed, infected, transplanted, antibody-opsinized, and ‘stressed’ cells. How NK cells achieve self-nonself discrimination requires an understanding of the molecular interactions they use to sense what is harmless or dangerous, and how these molecules assist in the decision to ignore or respond to a target cell under surveillance. Our lab primarily investigates MHC-independent receptor-ligand interactions involving the NKR-P1:Clr (Klrb1:Clec2) recognition system. This paired recognition system includes both stimulatory (NKR-P1A/C/F) and inhibitory (NKR-P1B/D/G) receptors that recognize up/downregulated Clr ligands on target cells. In addition, we recently developed an in vitro cell line model of ILC function (MNK-1/3, which resemble ILC1/3, respectively). This model allows for rapid analysis of gain/loss-of-function genetics directly in ILC by gene overexpression/editing/deletion. The overall goal of the lab is to advance understanding of how receptor-ligand interactions and transcription factor networks affect innate immunity to cancer, infection, and transplantation.

Publications and Awards

Back to Top